Submitted to: International Society of Sugar Cane Technologists Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 10/10/2004
Publication Date: 1/30/2005
Citation: Comstock, J.C., Miller, J.D., Schnell II, R.J., Ayala Silva, T. Sugarcane yellow leaf virus in the world collection of sugarcane and related grasses at Miami, Florida. Proceedings International Society of Sugar Cane Technologists 25:691-693. 2005. Interpretive Summary: The relatively high incidence of sugarcane yellow leaf virus (SCYLV) detected in sugarcane clones vegetatively maintained in the World Collection of Sugarcane and Related Grasses at the USDA-ARS Subtropical Horticultural Research Station in Miami may restrict distribution. Incidence varied by Saccharum species and may reflect differences in resistance in the Saccharum spp. S. spontaneum clones had the lowest incidence (5.6 %) while S. officinarum had the highest incidence (78.2 %) of SCYLV infection. S. spontaneum clones may offer a source of resistance to SCYLV.
Technical Abstract: The status of sugarcane yellow leaf virus (SCYLV) infection was determined in Saccharum clones maintained in field plots at the World Collection of Sugarcane and Related Grasses at the USDA-ARS Subtropical Horticultural Research Station at Miami, Florida to identify putative resistant and susceptible clones. SCYLV infected clones were considered susceptible and virus-free clones were assumed to be putatively resistant to SCYLV. Five leaves were collected from each clone from different plants in the 3 m plot. The SCYLV infection status was determined by the tissue blot immunoassay. The incidence of SCYLV infection varied among Saccharum spp. The incidence of SCYLV was 5.6 % for 252 S. spontaneum clones, 12.7 % for 55 S. barberi clones, 45.9 % for 37 S. sinense clones, 60.0 % for 55 S. robustum clones and 78.2 % for 325 S. officinarum clones evaluated. Of 175 Saccharum hybrid clones 70.9 % were infected. Several clones (Bandejarmain Hitan, Chunnee, Co 213, Co 285, POJ 100, POJ 213, and POJ 2878) that were used early in breeding programs were SCYLV infected; whereas, clones, Kassoer, Co 206, Co 281 and POJ 1499 were virus free. Both NCo 310 and NCo 376, two widely grown cultivars were infected. Ratings for the individual clones will be posted on the webcite: http://www.ars-grin.gov/ars/SoAtlantic /Miami/ngr/gallry01.htm. The low SCYLV infection found in the S. spontaneum clones suggests this group is the most resistant. The high susceptibility to SCYLV of the hybrid clones is consistent because a number of clones in their early pedigrees were susceptible.