Submitted to: Genbank
Publication Type: Germplasm Release
Publication Acceptance Date: 1/1/2004
Publication Date: 6/1/2004
Citation: He, G., Meng, R., Guo, B., Dang, P. 2004. Nucleotide sequences of 100 (GGC)n microsatellites in peanut. GenBank, National Center for Biotechnology Information Accession No. AY526357 to AY526456. Available: http://www.ncbi.nlm.nih.gov/dbEST. Interpretive Summary:
Technical Abstract: In U.S., peanut has become the second-most important legume crop, with the U.S. peanut crop having an average farm-gate value of over $1 billion in the 1990s. U.S. peanut production in 2003 was 4.14 billion pounds, and Georgia produced 1.86 billion pounds. Peanut is also a valuable source of protein and oil. However, research has revealed very limited genetic variation at the DNA level. Gene discovery and marker development are needed in cultivated peanut. The development of molecular markers should enable research to measure and exploit variation in the cultivated peanut species. Microsatellites, or simple sequence repeats (SSRs), are stretches of DNA consisting of tandemly repeated short units of 1-6 base pairs in length. The uniqueness and the value of microsatellites arise from their multiallelic nature, codominant inheritance, relative abundance, extensive genome coverage and simple detection by PCR. We have constructed microsatellite enriched libraries for sequences. Release of these DNA sequences of (GGC)n enriched simple sequence repeats to public database, GenBank, will promote the interest in utilization and development of microsatellite markers in cultivated peanuts. These markers are valuable in cultivar identification, genetic mapping, and molecular breeding. Identification of the genes associated with the resistance traits and development of molecular markers associated with the resistance will bring new diversity into peanut gene pools and expedite the development of commercial cultivars with the desired characteristics.