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Title: ASSOCIATION ANALYSIS FOR THE DISCOVERY OF SEED SIZE QTL IN SOYBEAN

Author
item Hofmann, Nicolle
item Yaklich, Robert
item SPECHT, J - UNIVERSITY OF NEBRASKA
item SONG, QIJIANG - UNVIERSITY OF MARYLAND
item Cregan, Perry

Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2003
Publication Date: 5/15/2003
Citation: Hofmann, N.E., Yaklich, R.W., Specht, J.E., Song, Q., Cregan, P.B. 2003. Association analysis for the discovery of seed size QTL in soybean [abstract]. BARC Poster Day. Abstract 13 p. 538.

Interpretive Summary:

Technical Abstract: Association analysis has been used to identify correlations between genetic variants and trait differences, especially complex diseases, in mammalian systems. Association analysis based on case-control tests compares marker allele frequencies between a case group and a control group from an existing population. A difference in frequency is indicative of a linked gene affecting the trait of interest. The objective of this work is to determine if this approach can be applied to plants, specifically soybean, a self-fertilizing plant species. Using seed size as the quantitative variable, single nucleotide polymorphisms (SNPs) will be used in genome scans of two sets of soybean germplasm lines. The seed size of the first set ranges from 4.2g to 10.0g, and the second ranges from 20.0g to 38.0g per 100 seeds. The germplasms were chosen based on balancing the two groups for maturity group, Asian origin, stem termination, and flower color. Initial SNP marker analyses will focus on regions of the genome where verified seed size QTL have been reported. In addition, a conventional QTL analysis of progeny from a cross between two Glycine max cultivars, Mercury (7.5 g/100 seeds) and Saturn (28.0 g/100 seeds) will be used with the intention of verifying a subset of QTL identified in the association analysis. Two replications of both the large and small seeded germplasm lines as well as 245 F4-derived F5 lines from Mercury x Saturn cross were planted in 2003 at the USDA-ARS farms in Beltsville, Maryland and the University of Nebraska at Lincoln for phenotypic analysis.