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ARS Home » Southeast Area » Miami, Florida » Subtropical Horticulture Research » Research » Publications at this Location » Publication #163373


item Heath, Robert
item Espada, Cyntia
item Vazquez, Aime
item Schnell, Elena

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 5/16/2004
Publication Date: 5/16/2004
Citation: Heath, R.R., Espada, C., Vazquez, A., Schnell, E.Q. 2004. Analytical Techniques for the Quantification of Ammonia and Putrescine. Meeting Abstract.

Interpretive Summary: See abstract only

Technical Abstract: Traps baited with food-based attractants are the primary tools used for detection and elimination of pest fruit flies population. Ammonia and polyamines, such as putrescine are among the chemical components that are emitted from liquid protein baits that are attractive to fruit flies, and formulations of these compounds are used in fruit fly traps. Abilities to quantify these chemicals are needed for both, research on the role of chemical release rate in lure efficacy for different species, and for use in quality control of lure formulation. The determination of release rates and concentrations of such amino compounds are very challenging from an analytical chemistry standpoint. Several methods have been developed in an attempt to quantify ammonia and putrescine with high precision. These include spectroscopic methods (such as Fourier Transform infra red detection [FTIR]), electrochemical methods (such as ammonia selective ion probes), and chromatographic methods (such as gas chromatography with nitrogen/phosphorous detector [GC-NPD]) have been used. This poster will compare such techniques on the basis of their limit of detection (LOD), reproducibility and sensitivity for analysis of ammonia and putrescine.