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ARS Home » Plains Area » Las Cruces, New Mexico » Range Management Research » Research » Publications at this Location » Publication #163126
Title: IN VITRO MASS CLONAL PROPAGATION AND HYPERHYDRICITY REVERSAL OF FOUR-WING SALTBUSH [ATRIPLEX CANESCENS (PURSH) NUTT.]

Author
item REYES, ISAAC - NEW MEXICO STATE UNIV
item HUBSTENBERGER, JOHN - NEW MEXICO STATE UNIV
item Barrow, Jerry

Submitted to: Congress on In Vitro Biology
Publication Type: Abstract Only
Publication Acceptance Date: 5/26/2004
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A successful, in vitro mass propagation system for cloning four-wing saltbush [Atriplex canescens (Pursh Nutt)] and rooting was achieved. In vitro propagated shoots of A. Canescens showed severe symptoms of hyperhydricity, a frequent physiological problem reported in micropropagation of A. canescens and other plant species. Hyperhydricity was exacerbated when cultured in airtight culture vessels and high ammonium concentrations in common media formulations and drastically reduced the quality of in vitro cultures. Hyperhydric shoots reverted to normal shoots by culturing on modified Murashige and Skoog basal medium and using larger culture vessels with vented closures. Resulting normal shoots significantly improved all aspects of micropropagation. One of the most relevant aspects of hyperhydricity reversal is the fact that only reverted shoots were able to root. Shoots were rooted in vitro by supplementing the culture medium with 2.46 FM of indolebutyric acid (IBA). Plants were successfully reestablished in soil transplanted and surviving in native sites.