Submitted to: Meeting Abstract
Publication Type: Book / chapter
Publication Acceptance Date: 2/16/2004
Publication Date: 4/30/2005
Citation: Lin, J.T., Mckeon, T.A. 2005. Hplc separation of the intact molecular species of some lipid classes. Meeting Abstract. pp. 373-396 (book chapter) Interpretive Summary: Lipids are structurally complicated and include many lipid classes. High performance liquid chromatography (HPLC) is the method of choice for the separation and identification of the molecular species of various lipid classes. In this book chapter, the HPLC methods for the separation and identification of the molecular species of important lipid classes were reviewed. This chapter is particularly useful in metabolic studies.
Technical Abstract: We have included in this chapter a broad array of HPLC systems separating the molecular species of six lipid classes, including phospholipids and glycolipids. The separation of the molecular species were usually by reversed-phase HPLC depending on the difference of fatty acyl chains with different polarities. The eluents used for the separation of the molecular species of various lipid classes are similar and one HPLC system usually can be used to separate the molecular species of many lipid classes. The elution orders of the molecular species of various lipid classes are usually the same. The eluent of the reversed-phase HPLC separation of the molecular species of phospholipids with charges requires silanol suppressing agent. We recommend the use of a volatile silanol suppressing agent such as NH4OH in the eluent, not only because LC-MS and ELSD can be used but also the eluent in HPLC fractions can be removed easily by a nitrogen stream.