Submitted to: Biotechnology Letters
Publication Type: Peer reviewed journal
Publication Acceptance Date: 3/2/2004
Publication Date: 3/2/2004
Citation: Kuykendall, L.D., Upchurch, R.G. 2004. Expression in sugar beet of the introduced cercosporin toxin export (cfp) gene from cercospora kikuchii, the causative organism of purple seed stain in soybean. Biotechnology Letters, 26:723-727. Interpretive Summary: A resistance gene known as CFP, that was isolated from the fungal pathogen Cercospora, was transferred into sugar beet plants. Sugar beet is susceptible to infection by Cercospora. The CFP gene was stably maintained within the chromosomal DNA of these plants. Both CFP messenger RNA transcripts and the CFP protein were expressed within the leaf cells of genetically engineered plants. Analysis of the relative susceptibility of CFP-transgenic and non-transgenic sugar beet plants is planned but will probably take several years to complete.
Technical Abstract: The Cercospora kikuchi cercosporin export gene, CFP, introduced into Beta vulgaris L. by conjugation with Rhizobium radiobacter, was stably maintained during vegetative propagation as verified by PCR using primers specific for the CFP gene. Transcriptional expression of the CFP gene in leaves was determined by RT-PCR using CFP-specific primers. CFP protein was detected using Western analysis with an affinity-purified polypeptide-specific antibody. Analysis of the relative susceptibility of CFP-transgenic and non-transgenic sugar beet plants is planned but will probably take several years to complete.