Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2004
Publication Date: 7/10/2004
Citation: Neill, J.D., Ridpath, J.F. 2004. Remodeling of cellular functions following infection with bovine viral diarrhea virus type 2 as indicated using serial analysis of gene expression. 23rd Annual Meeting of the American Society for Virology. Paper No. P14-2. Interpretive Summary:
Technical Abstract: Bovine viral diarrhea virus (BVDV) exists as one of two biotypes, BVDV type 1 or BVDV type 2. The most serious form of disease caused by BVDV, severe acute, is caused by a small number of BVDV type 2 strains. The determinants of virulence are unknown. To investigate virus-host interactions of the BVDV2 infection, serial analysis of gene expression (SAGE), a powerful sequence-based method to measure global gene expression, was employed to compare gene expression levels in noninfected MDBK cells and in MDBK cells infected with the virulent noncytopathic BVDV2 strain 1373. Comparison of the two libraries revealed a number of genes with altered transcription levels that could be placed into functional categories reflecting changes in the biochemical processes of BVDV2-infected cells. Showing decreased transcription were genes encoding several 14-3-3 proteins that function in intracellular signaling and participate in the regulation of a number of cellular processes. Expression of genes involved in protein translation and post-translational processing were generally upregulated. These included transcripts encoding ribosomal proteins, translation elongation factor 2, tRNA synthases and proteins that interact with nascent polypeptides during transport into the endoplasmic reticulum, Sec61-alpha homolog and translocating chain-associating membrane protein (TRAM). Additionally, transcripts encoding proteins that participate in vesical movement and targeting were more abundant in BVDV2-infected cells, including endobrevin (vesical associated membrane protein 8; VAMP8), and soluble NSF attachment protein alpha (alpha-SNAP). These findings provide support that BVDV2 infection results in altered transcription of genes that remodel cellular functions necessary for viral replication, maturation and release.