Submitted to: Plant Cell Tissue and Organ Culture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/20/2003
Publication Date: 7/1/2004
Citation: Guenzi, A.C., Scheets, K., Green, J.L., Fellers, J.P. 2004. Development and characterization of a nonmorphogenetic cell line of wheat (triticum aestivum l.). Plant Cell Tissue And Organ Culture. 78:23-28. Interpretive Summary: Some of the key research tools include cell culture lines. These are important to study gene function and cell response on the single cell level, then one can move up to a more complex organisms. The work that is reported is a wheat cell line that has been cultured for over ten years. They have lost their ability to regenerate new plants, however. the can serve as an important tool for studying the activities of various wheat genes. Previously, only corn cells were available. Our work has shown that this cell line has the same growth characteristics as the corn line, but will be more specific to wheat applications.
Technical Abstract: With the intention of making an immortal wheat (Triticum aestivum L.) cell line available for cellular and molecular biology research, this study was conducted to compare characteristics of a wheat cell line to those of the maize (Zea mays L.) Black Mexican Sweet (BMS) cell line and to compare protoplasts isolated from suspension cells of these cell lines. The wheat cell line was established from immature-embryo derived callus of the experimental line 'ND7532' and was conditioned for growth in suspension culture. For both cell lines, measurements of packed cell volume (PCV), fresh weight (FW), and dry weight (DW) were taken at 3 d intervals from suspension cultures. Measurements of FW of calli cultured from suspension cells of both cell lines were taken at 6 d intervals. The morphogenetic potential of the wheat ND7532 cell line was tested in both callus and suspension cultures using media promoting regeneration and/or organogenesis. Growth rates of ND7532 cells in suspension culture were comparable to those of BMS cells. However, relative growth rates of calli recovered from ND7532 suspension cells were slower than those of calli recovered from BMS suspension cells. The ND7532 cell line has very limited morphogenetic potential and has been maintained as rapidly growing callus tissue for eleven years. Yields of protoplasts from suspension cells of the two cell lines were comparable, though ND7532 protoplasts were typically smaller. The wheat cell line has is now designated ND7532-NM (nonmorphogenetic) and is available for cellular and molecular biology research.