Submitted to: Acta Horticulturae
Publication Type: Abstract only
Publication Acceptance Date: 4/15/2003
Publication Date: 7/21/2003
Citation: Tzanetakis, I.E., Halgren, A.B., Keller, K.E., Wintermantel, W.M., Martin, R.R. 2003. Two criniviruses are associated with the strawberry pallidosis disease. Acta Horticulturae. Interpretive Summary:
Technical Abstract: We have identified two closteroviruses belonging to the Crinivirus genus as potential causal agents of strawberry pallidosis disease. One is a newly described virus designated as Strawberry pallidosis associated virus (SPaV) while the second is Beet pseudo-yellows virus (BPYV). Both viruses are present in California, Eastern U.S., and may be present in other strawberry production regions as well. We have sequenced all the genes of both viruses and phylogenetic analysis revealed that the two viruses are more closely related to each other than any other virus in the genus. Transmission studies have identified Trialeuroides vaporariorum, the greenhouse whitefly, as a potential vector of the SpaV while studies involving other whitefly species are in progress. We have developed polyclonal antibodies against the recombinant coat protein of SPaV that have been used successfully in tissue blots but not ELISA. We are currently producing monoclonal antibodies to the virus and potentially to all Criniviruses.