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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #160379
Title: CHARACTERIZATION OF AN APHID-TRANSMITTED VIRUS ASSOCIATED WITH BLACK RASPBERRY DECLINE IN OREGON

Author
item HALGREN, A - OREGON STATE UNIVERSITY
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item Martin, Robert

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 4/18/2003
Publication Date: 6/6/2003
Citation: Halgren, A.B., Tzanetakis, I.E., Martin, R.R. 2003. Characterization of an aphid-transmitted virus associated with black raspberry decline in Oregon. Phytopathology. 93:S32.

Interpretive Summary: .

Technical Abstract: The objectives of this study were to characterize an unknown virus associated with decline in black raspberry (Rubus occidentalis) in Oregon. Mechanical and aphid transmissions from symptomatic R. occidentalis to Nicotiana benthamiana and aphid transmission to virus-free R. occidentalis have been successful. DsRNA extraction from symptomatic R. occidentalis revealed two major bands of about 8 and 9kb, suggesting a bipartite genome structure. Sequence information was obtained by cloning, using random primers, and a RT-PCR detection method was developed that enabled viral detection in several symptomless hosts, including R. idaeus, R. laciniatus, R. armeniacus, and 'Marion' blackberry (a Rubus sp. complex). This virus may be Black raspberry necrosis virus (BRNV), originally described in 1955 but poorly understood. However, grafting and aphid transmission do not induce the characteristic tip necrosis seen with BRNV. Partial sequence analysis of the genome reveals homology to the Strawberry mottle virus (SMoV).