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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Food Composition and Methods Development Laboratory » Research » Publications at this Location » Publication #160309


item Luthria, Devanand - Dave
item Vinjamoori, Dutt
item Noel, Kirk

Submitted to: American Oil Chemists' Society Meeting
Publication Type: Book / Chapter
Publication Acceptance Date: 2/10/2004
Publication Date: 5/1/2004
Citation: Luthria, D.L., Vinjamoori, D., Noel, K. 2004. Impact of moisture content, grinding and extraction technologies on assay of crude fat. In: Luthria, D.L. editor. Oil Extraction and Analysis: Critical Issues and Comparative Studeis. Champaign, IL: AOCS Press. p. 118-136.

Interpretive Summary:

Technical Abstract: Conventional breeding as well as transgenic approaches constantly strives in making improvements to quality traits such as increase in oil percentage and/or modification of oil composition. One of the key challenges faced by the industry is obtaining accurate, cost effective and rapid analysis of oilseeds/grains with enhanced quality traits such as total crude fat (oil) content or its modified oil composition. Reliable crude fat analysis is of paramount importance to oilseed businesses as monetary assessment in trade of such seeds is based on total oil values. Although several different primary and secondary technologies are available to determine crude fat content in oilseeds, there are significant variations in the results reported by different procedures. A comparative evaluation of different grinders (Mega-grinder, Knifetec, Cyclotec, Cemotec, Mikro Mill, UDY Grinder, Brinkmann-Retsch Grinding Mill) and commonly performed crude fat extraction methodologies (Accelerated Solvent Extractor, Supercritical Fluid Extraction, Ankom Batch Extraction, Automated Soxtec Extraction and classical Butt-tube) on the determination of total crude fat content in corn and soy seeds is presented. The results obtained clearly suggest a need for harmonization of official primary reference methods across different organizations (AOCS, AOAC, AACC, ISO, DGF, etc). This is vital for development of rugged calibrations for non-destructive, high throughput secondary procedures involving Near Infrared Transmittance, Near Infrared Reflectance/Imaging and Nuclear Magnetic Resonance Spectroscopy. Strategies aimed at harmonization of methods will aid in development of successful business opportunities and getting fair trade value for the quality enhanced traits in global market. Recommendations for developing secondary calibrations and performing inter-laboratories studies have also been presented.