Submitted to: International Conference on Methyl Bromide Alternatives and Emissions Reductions
Publication Type: Proceedings
Publication Acceptance Date: 9/14/2003
Publication Date: 11/2/2003
Citation: Loper, J.E., Press, C.M. 2003. Applications of genomics to biological control of soilborne diseases. International Conference on Methyl Bromide Alternatives and Emissions Reductions.
Technical Abstract: The complete genome of the biological control organism Pseudomonas fluorescens Pf-5 was sequenced. Strain Pf-5 was selected as a model biocontrol strain for a genomic sequencing project because of its success as a biocontrol agent, its use as a standard biocontrol strain by many researchers, and the body of knowledge that exists on the mechanisms by which Pf-5 suppresses soilborne plant disease. Genomic sequencing of Pf-5 began in March of 2002 at The Institute of Genomics Research, under the direction of Dr. Ian Paulsen, and was completed in September of 2003. Currently, the genome is being annotated to assign functions to as many genes in the genome of Pf-5 as possible. Completion of genome annotation is expected in spring of 2004. Post-genomics technology offers great promise for enhancing knowledge of biological control. To this end, we developed a low-density microarray for Pf-5 in which factors influencing the expression of genes required for biological control are being evaluated.