|Donoghue, Ann - Annie|
Submitted to: Southern Poultry Science Society Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2003
Publication Date: 9/29/2004
Citation: Nava, G.M., Tellez, G., Vincente, J.L., Bielke, L.R., Blore, P.J., Donoghue, A.M., Hargis, B.M. 2004. Novel lactobacillus culture demonstrates bacteriocin-like activity in vitro against multiple food borne pathogens and in vivo efficacy against SE [abstract]. Poultry Science. 83(Suppl 1):1762.
Technical Abstract: Mechanisms by which probiotic supplements affect the microecology of the intestinal tract are not well understood. Some effective probiotic bacteria produce bacteriocins, antimicrobial compounds which inhibit pathogenic bacteria. In the present investigation, several in vitro and in vivo studies were performed to demonstrate the antagonistic effect of a novel Lactobacilli probiotic culture (PHL-1) with wide in vitro antibacterial spectrum by bacteriocin-like activity. The two Lactobacilli present on the PHL-1 culture were isolated from different intestinal sections of healthy poults, and were identified as Lactobacillus casei and Lactobacillus cellobiosus by the MIDI Sherlock Microbial Identification System. Pure cultures of Lactobacillus casei and Lactobacillus cellobiosus were grown on MRS agar and were evaluated by agar plate diffusion for bacteriocin-like activity against pathogenic field strains of Salmonella enteritidis PT-13a (SE), Escherichia coli O2, Campylobacter jejuni, and Campylobacter coli. The two LAB present in PHL-1 culture showed clear inhibition zones against these intestinal pathogens in the agar diffusion technique. Independent in vivo experiments demonstrated significant reduction (83% at 7 days of age and 25% at 14 days of age compared to control) on SE cecal colonization in broiler chickens after prophylactic administration of PHL-1 at 1-3 days of age (P < 0.05). These studies demonstrate that the Lactobacillus bacteria produce bacteriocin-like activity against several food borne pathogens in vitro and efficacy against SE in vivo.