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ARS Home » Research » Publications at this Location » Publication #158052
Title: LESQUERELLA FENDLERI PROTEIN FRACTIONATION AND PROPERTIES

Author
item Wu, Ying Victor

Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: 5/9/2004
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Lesquerella fendleri is a promising new crop whose seed contains hydroxy fatty acid triglycerides with potential industrial uses. In addition to the oil, lesquerella seed has substantial amounts of valuable gums. Hexane defatted seed has more than 30% protein content. Defatted lesquerella meal was extracted sequentially with 0.5M sodium chloride (2x), water, 70% ethanol, and 0.1 N Sodium hydroxide (2x). Each sodium chloride extract was dialyzed against deionized water and centrifuged to separate water-soluble fraction (albumin) from salt-soluble fraction (globulin) before freeze drying. Ethanol extract and neutralized sodium hydroxide extracts (glutelin) were dialyzed against water and freeze dried. Albumin had highest lysine and sulfur amino acids per 16 g nitrogen among all the fractions analyzed. Glutelin and globulin accounted for the highest amount of protein nitrogen.