Skip to main content
ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Insect Behavior and Biocontrol Research » Research » Publications at this Location » Publication #157806
Title: UTILIZING IMMUNOASSAYS TO DETERMINE SYSTEMIC TOMATO SPOTTED WILT VIRUS INFECTION FOR ELUCIDATING FIELD RESISTANCE IN PEANUT

Author
item Murakami, M - UNIV OF FL
item GALLO-MEAGHER, MARIA - UNIV OF FL
item GORBET, D - UNIV OF FL
item Meagher, Robert - Rob

Submitted to: Crop Protection
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/20/2005
Publication Date: 3/1/2006
Citation: Murakami, M., Gallo-Meagher, M., Gorbet, D.W., Meagher Jr, R.L. 2006. Utilizing immunoassays to determine systemic tomato spotted wilt virus infection for elucidating field resistance in peanut. Crop Protection Journal. 25:235-243.

Interpretive Summary: Spotted wilt is a plant disease caused by tomato spotted wilt virus that is transmitted to plants by several species of thrips. It is a major disease on tobacco, tomato, and recently in the southeastern United States, peanuts. Management of the disease includes traditional breeding to select resistant peanut cultivars, and farming practices such as the time of year that peanuts are planted and the spacing between plants. This report focuses on research conducted by University of Florida scientists and a scientist at the Center for Medical, Agricultural and Veterinary Entomology, Gainesville, Florida. The effects of planting date, peanut cultivar, and plant spacing on infection and peanut yield were studied, as was how quickly the virus spreads within the peanut plant. Results suggested that a new cultivar, DP-1, planted in May and with a narrow plant spacing, produced the highest peanut yield tested. The virus moved from leaves to the root crown more quickly in the highly susceptible cultivar.

Technical Abstract: Three cultivars, SunOleic95R, Southern Runner, and DP-1, were evaluated for tomato spotted wilt virus (TSWV) resistance in replicated field plots at Marianna, FL, in 1998 and 1999. Treatments consisted of April and May planting dates with 7.6 or 15.2 cm space plantings. The presence of TSWV was determined by Enzyme-Linked Immunosorbent Assay (ELISA). SunOleic95R had the highest incidence of tomato spotted wilt, followed by Southern Runner and DP-1. TSWV was found in the root crown of SunOleic95R earlier than for Southern Runner and DP-1. DP-1 had the highest yield, followed by Southern Runner and SunOleic95R. May planting and a 7.6 cm plant spacing produced the highest yields regardless of peanut genotype.