Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2003
Publication Date: 7/1/2004
Citation: Li, S., Akan, D., Hartman, G.L., Band, M. 2004. Comparison of fusarium solani f. sp. glycines and saccharomyces cerevisiae genes using the affymetrix yeast genechip. Phytopathology; 2004.
Technical Abstract: Fusarium solani f. sp. glycines (Fsg) is in the same fungal class as Saccharomyces cerevisiae. To compare the genes of these two fungi, cDNA was synthesized by reverse transcription from total RNA extracted from a culture of Fsg. cRNA was synthesized from cDNA and labeled with biotin by in vitro transcription. After fragmentation, cRNA was hybridized to the Affymetrix GeneChip Yeast Genome S98 Array containing 9159 probe sets. The array was then scanned in an Agilent GeneArray Scanner and the MicroArray Suite was used to identify 'Present' or 'Marginal' genes. Approximately 4.8% of the yeast probe sets hybridized to Fsg cRNA indicating that few genes had sufficient homology to hybridize to S. cerevisiae. Using the NetAffx analysis,Fsg cRNAs hybridized with probe-sets associated with signal transduction, structural and ribosomal proteins, metabolism, cell division, and transport. In addition, Fsg cRNA hybridized to a gene that is similar to the plant PR-1 class of pathogenesis-related protein genes and three genes involved in multiple-drug resistance.