Submitted to: Soybean Research World Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 2/1/2004
Publication Date: 2/1/2004
Citation: Posada, M.L., Boore, J.L., Frederick, R.D. Comparative analysis of expressed sequences in the soybean rust pathogen phakopsora pachyrhizi. Soybean Research World Conference Proceedings which took place from 2/29/04 to 3/5/04.
Technical Abstract: Phakopsora pachyrhizi Sydow is an obligate fungal pathogen that causes the soybean rust disease. Unidirectional cDNA libraries were constructed in the plasmid pSPORT1, using mRNA isolated from three different stages of the life cycle of the pathogen. mRNA was isolated from P. pachyrhizi urediniospores germinating on a water surface for 16 h and from infected soybean leaf tissue at 6-8 and 13-15 days post inoculation. Intercellular hyphal growth and sporulation peak at 6-8 and 13-15 days, respectively. A total of 18,000 expressed sequence tags (ESTs) were generated from the three stage-specific cDNA libraries. The ESTs were analyzed for redundancy within and among the three libraries, and were classified according to putative function based on similarity to known proteins. These ESTs will provide insight into the biochemical and physiological processes that are necessary for infection, survival and reproduction.