Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/16/2004
Publication Date: 8/1/2004
Citation: Labeda, D.P., Kroppenstedt, R.M. 2004. Emendation of the genus Glycomyces and description of Glycomyces algerieniensis sp.nov., Glycomyces arizonensis sp. nov., and Glycomyces lechevalierae sp. nov.. International Journal of Systematic and Evolutionary Microbiology. 54:2343-2346. Interpretive Summary: Microorganisms have greatly benefited mankind by providing a vast array of natural products, such as antibiotics and enzymes, and by mediating useful bioconversions of other products even though the diversity of microbial species is still greatly underestimated. The genus Glycomyces was described with only 2 known species almost 20 years ago, and included one species that produced a novel antibiotic having anti-tumor potential. Only one additional new species has been described in the intervening period, although a number of tentative members of this genus were present in the Agricultural Research Service (ARS) Culture Collection having been isolated from nature by a new selective method or obtained from a private collection upon the retirement of the original curator. An evaluation of a set of putative Glycomyces isolates using a broad range of tests, including molecular genetic methods, biochemical analyses, and traditional physiological methods, confirmed the identity of several of the strains as Glycomyces and permitted the description of three new species. More importantly, this study also validated the effectiveness of the selective isolation procedure for obtaining strains of the genus Glycomyces, which were formerly considered extremely rare, which will provide researchers in biotechnology the ability to potentially exploit this genus as a source of novel antibiotics and other compounds.
Technical Abstract: A polyphasic taxonomic evaluation of presumptive strains of the genus Glycomyces held within the Agricultural Research Service (ARS) Culture Collection resulted in the discovery of three new species. Analysis of the whole cell sugar content of these new species, as well as of two species presently assigned to the genus revealed that the whole cell sugar pattern was different than reported in the formal description of the genus Glycomyces. The sugars present in all strains studied included ribose, xylose, mannose, and galactose rather than xylose and arabinose as reported in the description of the genus by Labeda et al., 1985. Moreover, the menaquinone patterns observed for the new species also deviated from the original genus description. The formal description of the genus Glycomyces is emended to reflect these new data. The new species proposed and described include Glycomyces algeriensis. NRRL B-16327T (= DSM 44727T), Glycomyces arizonensis NRRL B-16153T (=DSM 44726T), and Glycomyces lechevalierae NRRL B-16149T (=DSM 44724T).