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United States Department of Agriculture

Agricultural Research Service


item Saha, Badal

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2004
Publication Date: 4/1/2004
Citation: Saha, B.C. 2004. Endoglucanase from a newly isolated strain of Mucor circinelloides [abstract]. American Chemical Society. Paper No. BIOT 233.

Interpretive Summary:

Technical Abstract: In the USA, the production of fuel alcohol from corn starch reached about 2.1 billion gallons in 2002. Emphasis is to use lignocellulosic biomass as low-cost feedstock. One constraint is the high cost and low effectiveness of commercial cellulases. A newly isolated strain of the fungus, Mucor circinelloides (NRRL 26519), produces a complete cellulase (endoglucanase, cellobiohydrolase, and ß-glucosidase) enzyme system. The extracellular endoglucanase (EG) from this fungal strain was purified to homogeneity from the culture supernatant by ethanol precipitation (75%, v/v), CM Bio-Gel A column chromatography, and Bio-Gel A-0.5m gel filtration. The purified EG (specific activity 43.33 U/mg protein) is a monomeric protein with a molecular weight of 27,000. The optimum temperature and pH for the action of the enzyme are at 55 deg C and 4.0-6.0, respectively. The purified enzyme is fully stable at pH 4.0-7.0 and temperature up to 60 deg C. It hydrolyzed carboxymethyl cellulose and insoluble cellulose substrates to soluble cellodextrins. Comparative properties of this enzyme with other fungal EGs will be presented.

Last Modified: 10/16/2017
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