Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2003
Publication Date: 1/12/2004
Citation: Lu, J., Hunter, W.B., Dang, P.M., Huang, H., Leong, S. 2004. Identifying disease defense- and stress related genes in the grape Vitis shuttleworthii improving grape varieties an EST program. Plant and Animal Genome Conference.
Technical Abstract: Vitis shuttleworthii is one of the most disease and pest resistant grape species, which originated and is distributed in the southeast United States. It is highly resistant to: Pierce's Disease, Anthracnose, Black rot and Downy mildew diseases, which are the limited factors to grow Vitis vinifera grapes in this region. Clones of V. shuttleworthii have been extensively used by grape breeders in this region for developing disease resistant cultivars to combat diseases, pests, and environmental stresses. For these reasons, we selected V. shuttleworthii accession JL 2001 for our expressed sequence tag (EST) program for gene discovery and marker development. cDNA libraries were constructed from mRNA isolated from leaves and flowers harvested during anthesis. Phase I of this project produced 15,000 EST sequences. Sequence analysis revealed that 6% of the V. shuttleworthii ESTs are unique when compared to the existing Vitis vinifera NCBI data bases, and 1% of the ESTs did not find any homologous sequences among all plant ESTs reported in NCBI. Overall, approximately 12% of ESTs were related to disease/pest defense or stress tolerance genes. Contigs and full-length cDNA are under construction to study protein expression profiles. Meanwhile, we are developing SSR and SNAP markers from our EST data set. The genome-wide identification of disease/defense- and stress-related genes from Vitis shuttleworthii grapes will provide important genes and genetic markers for the viticulture industries world-wide to develop new disease resistant grape varieties.