Submitted to: Animal Genetics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 4/15/2004
Publication Date: 6/8/2004
Citation: Connor, E.E., Sonstegard, T.S., Ashwell, M.S., Bennett, G.L., Williams, J.L. 2004. An expanded comparative map of bovine chromosome 27 targeting dairy form QTL regions. Animal Genetics. 35:265-269. Interpretive Summary: Two regions of bovine chromosome 27 were previously identified as potentially containing genes contributing to the conformation trait, dairy form, in cattle. At present, the identity of genes located in these chromosomal regions is not known, preventing the selection of candidate genes controlling this trait. The purpose of this study was to improve the bovine-human comparative gene map so that positional candidate genes may be identified using the recently completed human genome sequence. The mapping of 12 genes to the cattle maps is described. The study improves the breakpoint resolution of the bovine-human comparative map and should improve the efficiency of candidate gene selection for the dairy form trait in cattle.
Technical Abstract: At present, the density of genes on the bovine maps is extremely limited and current resolution of the human-bovine comparative map is insufficient for selection of candidate genes controlling many economic traits of interest in dairy cattle. This study describes the chromosomal mapping of 12 selected gene-associated markers to bovine linkage and radiation hybrid maps to improve the breakpoint resolution in the human-bovine comparative map near two previously identified quantitative trait loci for the conformation trait, dairy form. Two novel regions of conserved synteny are described between the telomeric region of bovine chromosome 27 (BTA27) and human chromosome 3 (HSA3) p24 region and between the HSA4q34.1 region and BTA8. These data increase the number of genes positioned on the bovine gene maps, refine the human-bovine comparative map, and should improve the efficiency of candidate gene selection for the dairy form trait in cattle.