Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract only
Publication Acceptance Date: 10/15/2002
Publication Date: 1/15/2003
Citation: Narasimhamoorthy, B., Fritz, A.K., Gill, B.S., Brown Guedira, G.L. 2003. Advanced backcross qtl analysis of a synthetic hexaploid by winter wheat population. Plant and Animal Genome Abstracts. Interpretive Summary: Advanced backcross QTL analysis has been proposed as an approach for combining marker-based QTL discovery with variety improvement. The objective of the present study is to identify quantitative trait loci (QTLs) for yield and yield components in a population of 189 BC2F2:4 lines from a cross between the hard red winter wheat variety 'Karl 92' and the synthetic wheat line TA 4072 (T. turgidum ALTAR 84/Ae.tauschii WX 193). The parents were screened for polymorphism with 666 microsatellite markers with known chromosomal locations, of which 152 polymorphic markers were employed to genotype the population. Phenotypic data were collected for heading date, plant height, grain yield, tiller number per m2, kernels per spike, kernel weight, above ground biomass, harvest index, and grain volume weight. Lines were grown in three replications at Manhattan and Hutchinson, Kansas in the 2003-04 growing season. At both locations, BC2-derived lines were identified that exceeded the recurrent parents for grain yield and other traits. Single marker analysis and interval analysis identified QTLs for all traits except kernel weight. Of the 50 putative QTLs identified, the synthetic allele had a desirable effect at 34% of the loci. The present study provides insight into location of beneficial alleles from synthetic hexaploid wheat while providing agronomically desirable winter wheat germplasm for breeding purposes.
Technical Abstract: n/a