Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 4/11/2004
Publication Date: 6/1/2004
Citation: Smith, B.J., Hokanson, S.L., Hancock, J.F., Maas, J.L. 2004. Susceptibility of southern highbush blueberry clutivars to Botryosphaeria stem bight. Phytopathology. 94:S147 Interpretive Summary:
Technical Abstract: Stem blight, caused by the fungus Botryosphaeria dothidea, is a destructive disease of rabbiteye and highbush blueberries in the southeastern United States. The susceptibility of 20 southern highbush, two rabbiteye, and two highbush cultivars were compared using a detached stem assay. Fresh isolates of B. dothidea obtained from infected southern highbush blueberry plants were used as inoculum. Succulent, partially-hardened stems were surface disinfected, wounded by scraping away a section of bark, and inoculated by covering the wound with a mycelial agar block of B. dothedia and securing with parafilm wrap. The base of each stem was inserted into moistened, sterilized sand in a 150 x 25 mm tissue culture tube and incubated at 25C, 100 % RH for 30 days. Lesion length was measured after 15 days incubation. Cultivars with the shortest mean lesion length were classified as apparently resistant and included Pearl River, Emerald, Star, Sharpblue, Elliott, Misty, Bluecrisp, Darrow, Southmoon, Ozarkblue, Sapphire, and Brightwell. Cultivars with the longest lesions were classified as apparently susceptible and included Legacy, Gulf Coast, Cooper, Georgiagem, O'Neal, Reveille, Jubilee, and Magnolia. Tifblue, Windsor, Biloxi and Santa Fe were classified as tolerant.