Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/5/2003
Publication Date: 1/10/2004
Citation: Xu, S.S., Khan, K., Klindworth, D.L., Faris, J.D., Nygard, G. 2004. Chromosomal location of genes for novel glutenins and gliadins in wild emmer wheat. 12th Plant and Animal Genome Abstracts.p. 178.
Technical Abstract: The glutenin and gliadin proteins of wild emmer wheat, Triticum turgidum L. var. dicoccoides, have potential for improvement of durum wheat (T. turgidum L. var. durum) quality. The objective of this study was to determine the chromosomes controlling the high molecular weight (HMW) glutenin subunits and gliadin proteins present in three T. dicoccoides accessions (Israel-A, PI-481521, and PI-478742), which were used as chromosome donors in Langdon durum-Triticum dicoccoides (LDN-DIC) chromosome substitution lines. The three T. dicoccoides accessions and their respective LDN-DIC substitution lines were analyzed by SDS-PAGE, Urea/SDS-PAGE, and A-PAGE. The results revealed that all three T. dicoccoides accessions possess Glu-1A alleles that are the same as or similar to those reported previously. However, each T. dicoccoides accession had a unique Glu-B1 allele. PI-478742 had an unusual 1Bx subunit, which had mobility slightly slower than the 1Ax subunit in 12% SDS-PAGE gels. The subunits controlled by chromosome 1B of PI-481521 were slightly faster in mobility than the subunits of the Glu-B1n allele, and the 1By subunit was identified as band 8. The 1B subunits of Israel-A had similar mobility to subunits 14+16. Results from A-PAGE revealed that PI-481521, PI-478742, and Israel-A had eight, twelve, and nine unique gliadin bands, respectively, that were assigned to specific chromosomes. The identified glutenin and gliadin proteins in the LDN-DIC substitution lines provide the basis for evaluating their effects on end-use quality, and they are also useful biochemical markers for identifying specific T. dicoccoides chromosomes or chromosome segments.