Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 1/20/2003
Publication Date: 1/20/2003
Citation: Miftahudin, Chikmawati, T., Gustafson, J.P. 2003. Rice genome as a tool for cloning aluminum tolerance gene in rye (secale cereale l.)[abstract]. In: XI Plant and Animal Genome Conference Proceedings, January 11-15, 2003, San Diego, California. p.100. Interpretive Summary:
Technical Abstract: The use of aluminum (Al) tolerant varieties has been considered as a solution to overcome the problem of Al toxicity in acid soils, but development of such varieties demands basic knowledge of tolerance mechanisms, which are not well understood. Characterizing gene/s controlling Al tolerance in plants is a possible way to elucidate Al-tolerance mechanisms, however, none of the genes has been cloned. In an attempt to clone the Al-tolerance gene from rye, the most Al-tolerant grass species, we utilized a map-based-clone technique and the rye/rice syntenic relationship. Genetic analysis of a rye F6 RIL population revealed a single dominant gene (Alt3) located on the long arm of chromosome 4. Three AFLP markers flanking the gene and one RFLP marker co-segregating with Alt3 were identified. Syntenic analysis suggested that the Alt3 region was co-linier with a region of chromosome 3 in rice. Two sequence-tagged site (STS) flanking markers developed from a rice BAC clone facilitated the construction of a high-resolution map of the Alt3 region. By screening RFLP markers developed from the rice BAC clone and mapping them into an F2 population (2246 gametes) segregating for Alt3, we constructed a high-resolution map of the Alt3 region with one co-segregating marker and two flanking markers 0.1 cM apart. The Alt3 region spanning the flanking markers covers 10 kb region of the rice BAC clone that contains four candidate genes. One of the candidate genes is expressed only under Al stress in an Al-tolerant line.