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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #154233

Title: SPS Over Expression in Cotton and Its Effect on Lint Yield and Fiber Quality

item Scheffler, Jodi

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 9/8/2003
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Previous research indicated that cool night temperatures may cause lower cotton lint yields and immature fiber. Biochemical analyses of leaf (source) and fiber (sink) metabolism suggested that increasing sucrose phosphate synthase (SPS) activity at temperatures below 20 C should increase lint yields and improve fiber quality. To test this hypothesis, cotton (Gossypium hirsutum L., cv Coker 312) was transformed with the spinach SPS gene under the control of the CaMV 35S promoter. Transgenic plants were produced that expressed the gene and showed elevated levels of SPS activity. Field tests were conducted that included transgenic plants expressing the gene, not expressing the gene, Coker 312, and 6 commercial cultivars as checks. T3 and T4 progeny rows of the original transformants were tested over two years in replicated single row plots. The plots were evaluated for percent germination, flowering time, plant height, nodes to first boll, nodes above last boll, lint percent, cottonseed weight, 100 seed weight and average boll weight. Fiber samples were analyzed by HVI, AFIS, Stelometer and Arealometer. One transgenic line showed an increase in fiber strength over its transformation progenitor Coker 312, and was not significantly different than the commercial cultivars used as checks.