|La Rota, Mauricio|
Submitted to: Genome Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/4/2003
Publication Date: 8/1/2003
Citation: SORRELLS, M.E., LA ROTA, M., BERMUDEZ-KANDIANIS, C.E., GREENE, R.A., KANTETY, R., MUNKVOLD, J.D., MIFTAHUDIN, MAHMOUD, A., MA, X., ANDERSON, O.D. COMPARATIVE DNA SEQUENCE ANALYSIS OF WHEAT AND RICE GENOMES. GENOME RESEARCH. 2003. v. 13. p. 1818-1827. Interpretive Summary: The complete genome sequence of rice has been determined. One expectation is that once the rice sequence was known, it would be able to be used to assist in research of other cereals crops, such as barley, maize, oats, rye, and wheat, since some evidence exists that the genomes of the cereals crops have many similarities in the order of the genes along their respective chromosomes. However, no solid evidence to support or refute this hoped for similarity has been available. As part of a project to map the wheat genome in more detail than before, the present report compares the wheat map to the rice genome. The comparison of genes and their order in the two genomes shows that although there are significant similarities, the frequence of differences is high enough to seriously complicate the use of rice as a model for other major U.S. cereal crops such as barley, maize, and wheat.
Technical Abstract: The use of DNA sequence-based comparative genomics for evolutionary studies and for transferring information from model species to crop species has revolutionized molecular genetics and crop improvement strategies. This study compares 4485 ESTs that were physically mapped in wheat chromosome bins, to the public rice genome sequence data from 2251 ordered BAC/PAC clones using BLAST. A rice genome view of homologous wheat genome locations based on comparative sequence analysis revealed numerous chromosomal rearrangements that will significantly complicate the use of rice as a model for cross-species transfer of information in non-conserved regions. Supplementary information will be provided at the GrainGenes web site: http://wheat.pw.usda.gov/pubs/2003/Sorrells/.