Submitted to: NCB-ESA North Central Branch Entomological Society of America
Publication Type: Abstract Only
Publication Acceptance Date: 3/24/2003
Publication Date: 3/24/2003
Citation: Shukle, R.H., Johnson, A.J. 2003. Expression of hessian fly genes during compatible interactions with wheat. NCB-ESA North Central Branch Entomological Society of America.
Technical Abstract: We are identifying Hessian fly genes differentially expressed during compatible interactions with wheat. A pool of sequences differentially expressed in first-instar larvae is being identified by mRNA differential display. Homology searches will be used to identify known and unknown/novel sequences and genes potentially involved in the plant-insect interaction. To assess function of Hessian fly genes we are employing double-stranded RNA-interference (RNAi). From RNAi analysis of a Hessian fly glutathione S-transferase (GST) gene we propose a role for GSTs in the ability of Hessian fly larvae to deal with general defense responses of the host plant.