Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/13/2003
Publication Date: 11/10/2003
Citation: MCVICKER, J.K., TABATABAI, L.B. IDENTIFICATION OF A P5 COLONIZATION PROTEIN IN HAEMOPHILUS PARASUIS. RESEARCH WORKERS IN ANIMAL DISEASES CONFERENCE PROCEEDINGS. 2003. Abstract #115P.
Technical Abstract: In humans, the outer membrane protein P5 from Haemophilus influenzae has been shown to aid colonization in the nasopharynx by binding to CD66 on the surface of mucosal epithelial cells. The intent here was to determine if Haemophilus parasuis contained a homolog of the P5 protein. Using outer membrane protein P5 primers designed from the H. influenzae database, we were able to detect a single PCR product in a H. parasuis field isolate. The PCR product was transformed into a competent E. coli strain and sequenced. DNA sequence analysis revealed homology to the H. influenzae P5 protein. Immunoblots were performed with 15 H. parasuis reference strains using a P5 monoclonal antibody. A 48 kDa protein was identified in all but one virulent reference strain, whereas a 55 kDa protein was identified in all but one non-virulent reference strain. Mass spectrometry and N-terminal sequencing were used to confirm the identity of the P5 protein in a virulent field isolate and reference strains.