Submitted to: Mid Atlantic Plant Molecular Biology Society Conference
Publication Type: Abstract Only
Publication Acceptance Date: 7/23/2003
Publication Date: 7/23/2003
Citation: Khan, R., Macdonald, M.H., Alkharouf, N., Ben Chouikha, I., Beard, H.S. 2003. Interactions between defense signaling pathways in soybean plants [abstract].. Mid Atlantic Plant Molecular Biology Society Conference. p.31.
Technical Abstract: Soybean cyst nematode (SCN) is one of the major pathogens of soybean in the US causing millions of dollars worth of damage each year. The defense response of soybean to SCN is a multigenic trait and varies depending upon the genotypes of soybean and SCN. We looked at the gene expression profile of approximately 6000 genes in nematode (SCN), salicylic acid (SA), jasmonic acid (MeJA) and ethylene (E) treated SCN-resistant soybean plants. Genes that were commonly regulated by two or more treatments were identified. Crosstalk between nematode induced, SA, MeJA and E-activated pathways was observed. The expression of a few genes of these different defense pathways was also determined by qRT-PCR and Northern hybridization to validate the expression results observed from microarray analysis. To test if turning on these pathways was providing any protection against the invading nematode, we induced the SA and JA- pathways by treating the plants with these chemical compounds in an SCN-susceptible soybean cultivar before challenging it with the nematodes. Our results suggest that stimulation of these pathways prior to infection by nematodes causes a reduction in the infection. Overall there appears to be interactions and coordination between the different defense signaling pathways to ward off the invading pathogen