Submitted to: Maize Genetics Cooperation Newsletter
Publication Type: Research notes
Publication Acceptance Date: 1/31/2003
Publication Date: 7/18/2003
Citation: GOLDMAN, S.L., BLAKEY, C.A., FRUTIGER, K., DEWALD, C.L., SOKOLOV, V.A. TRIPSACUM STAGE- AND PLOIDY-SPECIFIC FLORAL CDNA LIBRARIES. MAIZE GENETICS COOPERATION NEWS LETTER. 2003. V. 77. P. 72-73. Interpretive Summary:
Technical Abstract: Using the pilot project designed at Ball State University(BSU), Muncie, IN, by Blakey et al (MNL 77), a set of five out of six possible stage-specific cDNA libraries were constructed at the Univ. of Toledo using the SMART™ PCR cDNA Synthesis Kit (CLONTECH Cat# K1052-1) at the University of Toledo. The original RNA was isolated in 1998 by Blakey et al (MNL 77) on-site in Woodward, OK, according to the Trizol™ RNA isolation protocol provided by Sigma. The materials were quantitated at BSU and half shipped to U. Toledo. Each library was labeled according to the staged tissue from which the RNA was isolated: "E" early diploid (E2) or early tetraploid (E4), "M" middle diploid (M2), and "L" late diploid (L2) or late tetraploid (L4). The only library that was not obtained at this time was the "M" middle tetraploid (M4) library. Twenty-five cDNAs from these libraries were shipped to the DNA Core Facility, Ohio State University, Columbus, OH, for sequencing. Sequence data analysis of clones from the E2 and M2 libraries indicates that the libraries include gene that function as a serine threonine protein kinase and ribosomal protein S1Sa mRNA respectively. Clones from the E4 and L2 libraries have resulted in chloroplast gene sequences, while a clone from the L4 library was considered novel at the time of this article.