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ARS Home » Southeast Area » Little Rock, Arkansas » Microbiome and Metabolism Research Unit » Research » Publications at this Location » Publication #151300
Title: LC/MS/MS ANALYSIS OF LYSOPHOSPHOLIPIDS ASSOCIATED WITH RICE PROTEIN ISOLATE

Author
item FANG, NIANBAI - UAMS
item YU, SHANGGONG - UAMS
item BADGER, THOMAS - 6251-10-00

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 1/10/2003
Publication Date: 3/17/2003
Citation: FANG, N., YU, S., BADGER, T.M. LC/MS/MS analysis of lysophospholipids associated with rice protein isolate. JOURNAL OF FEDERATION OF AMERICAN SOCIETIES FOR EXPERIMENTAL BIOLOGY. 2003. v. 17(5). p. A768. Abstract No. 459.12.

Interpretive Summary: In order to evaluate the effects of lysophospholipids on human health, researchers need to know how much and what kinds of lysophospholipids are consumed in rice protein isolates-foods. This paper reports two results: 1) Lysophospholipids are important biosynthetic precursors of bioactive phospholipids and have biological activity in their own right. However, little information is available regarding the direct characterization of individual lysophospholipids from complex matrices without multistep purification and separation process. The LC/MS/MS method developed in the present study was demonstrated to be well-suited for the rapid on-line characterization of lysophospholipids from complex matrices. 2) Rice protein isolate has been reported to provide potential health benefits in animal models, including reduction of serum cholesterol and decreased incidence of chemically-induced mammary cancers. There are, however, no published data on potential bioactive compounds in rice protein isolate that could account for these findings. Using these LC/MS/MS conditions, twenty-eight lysophospholipids including 12 lysophosphatidylethanolamines, 12 lysophosphatidylcholines and 4 lysophosphatidylglycerols were identified to be associated with rice protein isolate. Further studies are ongoing to determine which of the have biological functions and to determine the mechanisms by which they act to affect health.

Technical Abstract: Lysoglycerophospholipids (lyso-PLs) are important cell signaling and growth factor molecules experimentally linked to cardiovascular function and disease, cancer and neurological disorders. Rice protein isolate (RPI) has been reported to provide potential health benefits in animal models, including reduction of serum cholesterol and decreased incidence of chemically-induced mammary cancers. There are, however, no published data on potential bioactive compounds in RPI that could account for these findings. In the present study, RPI extracts were analyzed using an LC/MS/MS method. The method was first developed using commercially available standard mixtures. Under these LC/MS/MS conditions, lyso-PL compounds could be separated by LC and yielded unique fragments and neutral losses in the electrospray ionization mass spectrometry with both positive- and negative-ion mode, quite indicative of the polar headgroups for different subclasses. Also, the most widely used commercially available standard mixture of sn-2 lyso-PL was found to be a mixture of regioisomers (sn-1 and sn-2) by their different LC-retention behaviors, as well as diagnostic MS data. Twenty-eight lyso-PL components were identified to be associated with RPI. The method was demonstrated to be well-suited for the rapid on-line characterization of lyso-PLs from complex matrices.