Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2003
Publication Date: 11/15/2003
Citation: Brown, R.L., Chen, Z.Y., Menkir, A., Cleveland, T.E. 2003. Proteins associated with aflatoxin-resistance in maize lines from a west african breeding program [abstract]. Phytopathology. 93:S12.
Technical Abstract: Several U.S. maize lines resistant to Aspergillus flavus infection/aflatoxin production were incorporated into maize germplasm from Central and West Africa. These African lines had been selected for resistance to various fungal ear rots, and had preliminarily demonstrated aflatoxin-resistance as well. Lines produced from these crosses have undergone selfing and selection through 4 generations. Seed from S4 lines were investigated for aflatoxin accumulation using the laboratory-based kernel screening assay (KSA). Lines with the same parental background but differing in aflatoxin levels (near-isogenic) were then subjected to proteome analysis to identify constitutively-expressed proteins associated with resistance. Previous work has shown enhanced constitutive protein expression to be a differentiating factor between resistance and susceptibility. Results of these investigations will be discussed.