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ARS Home » Research » Publications at this Location » Publication #151068
Title: MELEAGRIS GALLOPAVO PREPROGHRELIN MRNA, COMPLETE CDS

Author
item Richards, Mark
item Poch, Stephen

Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 7/2/2003
Publication Date: 7/26/2003
Citation: Richards, M.P., Poch, S.M. 2003. Meleagris gallopavo preproghrelin MRNA, complete CDS [abstract]. GenBank. Accession No. AY333783.

Interpretive Summary:

Technical Abstract: A portion of the preproghrelin gene was isolated, sequenced and characterized from turkey proventriculus tissue using primer-directed reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of 5' and 3' cDNA ends (5' and 3' RACE). Direct sequencing of overlapping PCR-generated DNA fragments identified and confirmed a total of 869 contiguous bases corresponding to the preproghrelin mRNA (cDNA) including the complete coding region, and the 5'- and 3'-untranslated regions. From the nucleotide sequence, a preproprotein consisting of 116 amino acids was predicted that showed homology (i.e., identities and positive substitutions) of 67%, 64%, and 67% with corresponding preproghrelin amino acid sequence data previously reported for human, pig, and mouse, respectively. The predicted amino acid sequence demonstrated complete conservation of a ghrelin N-terminal 'active core' sequence (GSSF, amino acids 24-27 of the preproprotein) that includes a serine (S) residue (amino acid 26 of the preproprotein) shown previously to be a modification site for acylation by n-octanoic acid that is important for the binding of ghrelin to its receptor and its subsequent bioactivity. This sequence data will be useful in studying the structure, expression and function of the avian ghrelin gene, and in determining the role of ghrelin in the regulation of appetite and energy balance in commercial poultry species.