Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/3/2003
Publication Date: 11/9/2003
Citation: Purdy, C.W., Layton, R.C., Straus, D.C. 2003. Effects of mycotoxins in weanling goats given by transthoracic injection. Research Workers In Animal Diseases Conference Proceedings. p. 160
Technical Abstract: Mycotoxin preparations were made from each of three fungi (Mucor ramosissimus, Trichoderma virdiae, and Stachybotrys chartarum). Each fungus was grown on 30 Sabouraud dextrose agar plates for 14 days at 25C. One-hundred percent methanol (20 ml/plate) was used to extract both hyphae and spores. A course filter was used to remove the larger debris, and then a 0.22 mm polyvynilidene fluoride filter was used to remove the spores. The methanol was evaporated under a fume hood and the product of 30 plates per fungus was mixed with 400 ml of sterile water. Product from each fungus was freeze-dried to 1g. The product from each fungus was rehydrated with 10 ml of sterile saline. Twenty-four weanling Boar-Spanish cross goats were randomized into four groups (Saline control, n=6; Mucor, n=6; Trichoderma, n=6; and Stachybotrys, n=6). Each goat was tranquilized (0.22 mg xylazine/kg), clipped and the skin sanitized at the site of injection. One ml (100 mg) of the appropriate product was given by transthoracic injection into the mid-portion of the posterior lobe of the left lung. Three days following the injection into the lungs all animals were given an overdose of barbiturates and then necropsied. Injection site consolidated mean lesion volumes were measured with calipers: Controls, 4.5 mm3, Mucor group, 2413 mm3, Stachybotrys group, 1664 mm3, and Trichoderma group, 7064 mm3. The Mucor and Trichoderma mean lesions scores were significantly (P < 0.05) larger than the controls as measured by Dunnett's T test. The goats developed lung consolidation as a result of exposure to the transthoracic injection of the 3 mycostoxins.