Submitted to: Council for Study of Viruses and Virus Diseases of the Grapevine
Publication Type: Proceedings
Publication Acceptance Date: 9/12/2003
Publication Date: 9/20/2003
Citation: Lima, M.F., Alkowni, R., Rowhani, A., Uyemoto, J.K., Golino, D., Renault-Spilmont, A.S. 2003. Genomic study of grapevine rupestris stem pitting associated virus-like isolates. Council for Study of Viruses and Virus Diseases of the Grapevine. Interpretive Summary: Two different sources contained molecularly distinct isolates of Grapevine rupestris stem pitting associated virus (GRSPaV) when compared to sequences reported for type isolate. Specific primer sets will be designed to amply and identify each isolate, which should assist in our effort to correlate disease etiology with a specific isolate of GRSPaV.
Technical Abstract: Grapevine rupestris stem pitting associated virus (GRSPaV) is a component of rugose wood complex. During vineyard surveys, diseased grapevines were collected and tested by RT-PCR using a panel of primers specific for several grapevine viruses. From these assays, two different GRSPaVs were detected. One isolate was associated with a graft-union disorder in Syrah grapevine and another showing severe rootstock stem lesions grafted with Pinot noir scion. The respective dsRNAs were purified, amplified, cloned, sequenced and the sequences analyzed using the Wisconsin GCG software package and the National Center for Biotechnology Information. Nucleotide and amino acid sequences for both isolates showed identities, respectively, of 70 or 89% and 81 or 95% compared to GRSPaV type. With the Syrah isolate, the capsid protein region showed identities of 82 and 91% in nucleotide and amino acid sequences to GRSPaV type. These significant variations among the isolates will enable us to design primers for the detection and identification of specific virus isolates and help in determining disease symptoms with virus isolate.