Submitted to: Annual Beet Sugar Development Foundation Research Report
Publication Type: Other
Publication Acceptance Date: 4/1/2003
Publication Date: 5/1/2003
Citation: MCGRATH, J.M. DEVELOPMENT OF RECOMBINANT INBRED LINES IN BETA. 2002 ANNUAL BEET SUGAR DEVELOPMENT FOUNDATION RESEARCH REPORT. 2003. p. D28.
Technical Abstract: A strategy was initiated to capture genetic diversity of self-incompatible, open-pollinated USDA-ARS releases, wild species, and crop relatives (fodder, table beets, and chard) in a self-fertile genetic background. Over 100 self-fertile hybrid populations have been made to date. The primary goal is to develop Recombinant Inbred Lines (RILs) from representatives from each of these hybrids from F1 plants that have shown good field vigor as well as high seed yield. We used a rapid cycling strategy to advance 1.5 generations per year (e.g. growth in small, deep pots for six weeks, then 12 weeks of vernalization, followed by placing a bag over each small plant to enforce inbreeding, which yielded 5 to 50 seeds per plant) and will obtain F6 seed by single-seed-decent within four years. 50 RIL families, each with 100 individuals each, representing the diversity of beet germplasm currently among experimental hybrids described above will be targeted for synthesis. In 2002 using this system, one family has been advanced to the F5, two other families have been advanced to the F4, 27 other families are being advanced to the F3, and 22 are being advanced to the F2. The final result will be a set of 5,000 inbred lines covering the major agronomic traits to be released to the National Genetic Resources System (i.e. GRIN) for general distribution. These populations will be an invaluable resource for beet geneticists and others.