Submitted to: International Symposium of Molecular Breeding of Forage Turf
Publication Type: Abstract Only
Publication Acceptance Date: 3/2/2003
Publication Date: 5/18/2003
Citation: Krishnaramanujam, R., Read, J.C., Reinert, J.A., Burson, B.L., Hussey, M.A. 2003. Molecular mapping of fall armyworm (Lepidoptera: Noctuidea) resistance genes in Texas bluegrass [abstract]. 3rd International Symposium of Molecular Breeding of Forage and Turf. p. 16.
Technical Abstract: Fall armyworm, Spodoptera frugiferda (J.E. Smith) is a destructive pest that inflicts considerable damage on several important grasses, including Texas bluegrass (Poa arachnifera Torr.) an important turf grass in the southern United States. However, sources of resistance to the insect are not available in this grass. A breeding program was initiated to develop armyworm resistant lines by using Kentucky bluegrass (Poa pratensis L.), a closely related species, as the source of resistance. Crosses were made between heterozygous, outcross parents: susceptible Texas bluegrass line (TBPC 25-1125-88) and resistant Kentucky bluegrass cultivar 'Mystic'. The F1 hybrids segregated for resistance and susceptibility and were used for mapping. Bulk segregant analysis and amplified fragment length polymorphisms (AFLPs) markers were used to identify and map the resistance genes. More than 200 fluorescent labeled Pst/Mse I AFLP primer combinations were used to detect the polymorphic markers. Only 32 primer combinations were polymorphic between the bulks and revealed 53 polymorphisms. Forty three were in the resistant parent and bulk and the remaining 10 were in the susceptible parent and bulk. The polymorphic AFLP primer combinations were tested in mapping populations, and the results will be discussed.