Submitted to: Annual Beet Sugar Development Foundation Research Report
Publication Type: Other
Publication Acceptance Date: 4/1/2003
Publication Date: 5/1/2003
Citation: GUNASEKERA, S., BUGHRARA, A., MYERS, S., MCGRATH, J.M. HYDROGEN PEROXIDE CONCENTRATION IN STRESS GERMINATED SUGAR BEET SEEDS. 2002 ANNUAL BEET SUGAR DEVELOPMENT FOUNDATION RESEARCH REPORT. 2003. p. D33.
Technical Abstract: Screening large quantities of germplasm for production of hydrogen peroxide evolution during germination is required to evaluate the contribution of this mechanism to improved emergence and stand establishment. Previous results have shown that evolution of hydrogen peroxide is correlated with expression of a germin-like protein with putative oxalate oxidase activity, and this activity only occurs in the high emerging USH20 under laboratory stress at room temperature. A rapid test is needed to screen additional germplasm. Results from a new direct measure of hydrogen peroxide on single seeds using a fluorescent dye developed for this purpose were consistent with results from more laborious methods, and this new procedure allowed evaluation of the timing of hydrogen peroxide evolution as well. Future work will be to carry out a germplasm survey with different sugar beet lines to test for correlations between H2O2 concentration in germinating seedlings and their emergence in the field.