Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/19/2003
Publication Date: 8/1/2003
Citation: CEPICA, S., ROHRER, G.A. LINKAGE AND RADIATION HYBRID MAPPING OF THE PORCINE PIK3R1 GENE TO CHROMOSOME 16. ANIMAL GENETICS. 2003. v. 34. p. 313. Interpretive Summary: The phosphatidylinositol 3-kinase (PI3-kinase) enzyme plays an important role in energy metabolism. This enzyme has a regulatory and a catalytic subunit. One of the regulatory subunits of PI3-kinases (PIK3R1) was mapped in the pig genome in this study to chromosome 16. Mapping of PIK3R1 to SSC16 is in agreement with comparative mapping data. For its physiological role the PIK3R1 gene could be considered as a candidate gene for fat accumulation and food intake. However, so far no QTLs for these traits have been detected on SSC16.
Technical Abstract: A fragment of the porcine PIK3R1 (phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)) gene encompassing parts of exons 10 and 13 and intervening introns and exons was amplified. The amplicon was sequenced and the sequence data obtained matched exons 11 and 13 of the human sequence AC016564 with identity of 88% (142/161 nucleotides) and 89% (86/96 nucleotides), respectively. A biallelic polymorphism was detected with MvaI. Mendelian inheritance was confirmed in the USDA-MARC reference pedigree and allele frequencies in unrelated animals of eight pig breeds were determined. Based on 18 informative meioses the PIK3R1 marker was localized on the USDA-MARC linkage map to SSC16 position 40.1 Kosambi cM at the same location as SW81, SW382, SW1090 and SW1341.Radiation hybrid (RH) mapping was performed with the use of the INRA-University of Minnesota porcine Radiation Hybrid panel. Two-point analysis performed on the IMpRH Server (http://imprh.toulouse.inra.fr) showed the most significant linkage to SW1341 (LOD = 5.73, 56 cR) and together with this marker forms a new linkage group on the first porcine RH map.