Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/10/2003
Publication Date: 11/11/2003
Citation: KATO, K.K., PALMER, R.G. MOLECULAR MAPPING OF FOUR OVULE LETHAL MUTANTS IN SOYBEAN. THEORETICAL AND APPLIED GENETICS. 2003. v. 108. p. 577-585. Interpretive Summary: Many developmental steps are necessary to have normal male and female development in plants. Any error in these steps can result in partial sterile or complete sterile plants. Four changes (mutations) were identified in soybean that resulted in female partial-sterile plants with intact full male fertility. Our objective was to identify the genetic location of these four mutations. The four mutations occurred at different locations in the soybean genetic map. The location of these female partial-sterile mutations contributes to our knowledge of normal soybean sexual development. Such information assists plant breeders to produce better soybean varieties that contribute to better food and feed uses of soybean and soy products.
Technical Abstract: Four soybean ovule lethal mutants, PS-1, PS-2, PS-3, and PS-4, were identified as female partial-sterile mutants from a gene tagging study. The four mutants had been classified into two mutation classes, (1) PS-1; sporophytic mutation affects sporophytically expressed genes, and (2) PS-2, PS-3, and PS-4 mutants; female gametophyte-specific mutations affect gametophytically expressed genes and are transmitted through the male but not the female gametes. Molecular mapping demonstrated that these four mutant genes and previously reported female-partial sterile gene, Fsp1, are located independently on soybean molecular linkage groups (MLG-) using simple sequence repeat (SSR) markers. PS-1, designated as Fsp2 and Genetic Type Collection number T364, is located MLG-C2. PS-2, designated as Fsp3 and Genetic Type Collection number T365H, is located MLG-A2. PS-3, designated as Fsp4 and Genetic Type Collection number T366H, is located on the terminus of MLG-F. PS-4, designated as Fsp5 and Genetic Type Collection number T367H, is located MLG-G. SSR markers adjacent to Fsp3, Fsp4, and Fsp5 were distorted from a 1 : 2 : 1 ratio, and fit a 1 : 1 ratio. The segregation distortions of SSR markers adjacent to Fsp3, Fsp4, and Fsp5 are in support of male but not female transmission of the Fsp3, Fsp4, and Fsp5 gametes.