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United States Department of Agriculture

Agricultural Research Service


item Jia, Yulin
item Wang, Zhonghua
item Redus, Marc
item Singh, Pratibha
item Correll, James
item Lee, Fleet
item Valent, Barbara
item Xia, Yingwu
item Rutger, J

Submitted to: International Plant Protection Congress
Publication Type: Abstract Only
Publication Acceptance Date: 5/11/2003
Publication Date: 5/11/2003
Citation: Jia, Y., Wang, Z., Redus, M.A., Singh, P., Correll, J., Lee, F.N., Valent, B., Xia, Y., Rutger, J.N. 2003. Development of rice blast pi-ta resistance gene markers for marker-assisted selection breeding [abstract]. 15th International Plant Protection Congress Proceedings. p. 262.

Interpretive Summary:

Technical Abstract: Molecular characterization of approximately 48 plant resistance (R) genes has advanced the ability to engineer the effective resistance against pathogens. Knowledge of DNA sequences of susceptible haplotypes of R genes not only enhances the understanding of R gene evolution but also can be used to identify nucleotides that distinguish the resistant counterpart. These unique DNA sequences can be used to develop R gene specific primers to amplify portions of R gene as marker for a particular R gene for marker-assisted selection (MAS). Rice blast is one of the most destructive rice diseases worldwide. The rice blast Pi-ta resistance gene is one of those R genes characterized at the molecular level. One resistant Pi-ta and three susceptible pi-ta haplotypes were identified from rice germplasm. An alignment of DNA sequences of these Pi-ta haplotypes identified several nucleotide substitutions and these nucleotide differences were used to develop PCR based markers for stacking the Pi-ta gene into advanced rice breeding lines by MAS. Progress on development of codominant Pi-ta gene markers suitable for use in high-throughput semi-automated genotyping machines will be presented.

Last Modified: 06/27/2017
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