Submitted to: International Congress of Plant Molecular Biology
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/2003
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: Fifty percent of the grain legumes consumed worldwide are beans (Phaseolus sp.). Common bean (P. vulgaris) is economically and nutritionally important as a primary source of protein in the human diet in several countries. That makes common bean an important candidate for genomic studies. An international consortium called Phaseomics (Phaseo[lus] [geno]mics) has been formed to establish the necessary framework of knowledge and materials for the advancement of bean genomics. Phaseomics includes around 80 scientists from 20 different countries. A major goal of phaseomics is to generate new common bean varieties that are not only suitable for but also desired by the local farmer and consumer communities. As part of the global project of Phaseomics, the research efforts from our group are oriented toward 1) establishing an efficient genetic transformation system for P. vulgaris and 2) sequencing ESTs from different bean cDNA libraries and performing expression analyses. In collaboration with C. P. Vance (USDA-ARS), approximately 3,000 ESTs were sequenced from a common bean root nodule cDNA library. The source of the RNA for library preparation was 15 day old nodules of P. vulgaris cv Negro Jamapa 81, induced by Rhizobium tropici CIAT899. Clones were sequenced from the 5'-terminus and analyzed by BLASTX. The ESTs were grouped into four main functional categories: metabolism (25%), development (24%), interaction with the environment (21%), and unknown function (30%). Expression will be measured via macro- and micro-arrays. Other cDNA libraries have been prepared from young pods and from P-limited roots of P. vulgaris cv. negro Jamapa plants.