Submitted to: Biotechnology for Fuels and Chemicals Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/7/2003
Publication Date: 5/7/2003
Citation: QURESHI, N., MADDOX, I.S. PRODUCTION OF BUTANOL FROM CONCENTRATED LACTOSE/WHEY PERMEATE USING CLOSTRIDIUM ACETOBUTYLICUM AND REMOVAL BY PERSTRACTION. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS. 2003. PAPER NO. 3-10. Interpretive Summary:
Technical Abstract: Acetone-butanol-ethanol (ABE) were produced from whey permeate medium, supplemented with lactose, in a batch reactor using Clostridium acetobutylicum P262, coupled with ABE removal by perstraction. ABE (87.2 g/L) were produced from lactose (277 g/L) at a yield of 0.43 and productivity of 0.22 g/L.h. These results are superior to ABE batch fermentation where a maximum ABE concentration of 20 g/L is achieved. Silicone tubing (membrane area 0.1130 m2 based on inside diameter) was used as the perstraction membrane while oleyl alcohol was used as the perstraction solvent. Removal of ABE by perstraction was faster than their production in the reactor, and the maximum concentration of ABE in the oleyl alcohol was 7.5 g/L. However, the fermentation ceased prior to complete lactose utilization, probably due to back-diffusion of oleyl alcohol into the fermentation medium. Hence, perstraction compares unfavorably with gas stripping as the product removal technique in integrated batch fermentation/product removal processes utilizing high sugar concentrations.