Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/31/2003
Publication Date: 8/1/2003
Citation: Chen, J., Civerolo, E.L., Jarret, R.L., Van Sluys, M.A., De Oliveira, M.C. 2003. Characterization of a Xylella fastidiosa field strain on the genomics basis [Abstract]. Phytopathology. 93:S16.
Technical Abstract: Five randomly amplified polymorphic DNA (RAPD) fragments from a Florida strain of Xylella fastidiosa (Xf) causing Pierce's disease (PD) in grapevine were sequenced and used to search the GenBank database including the genome sequences of four Xf strains causing PD (PD-Temecula), almond leaf scorch (ALSD-Dixon) and oleander leaf scorch (OLSD-Ann-1) from California, and citrus variegated chlorosis (CVC- 9a5c) from Brazil. Four of the five RAPDs were most similar to PD-Temecula, indicating that Xf-PD strains from Florida and California were highly homogeneous. CVC-9a5c was the most distant to the Florida PD strain. The discrepant RAPD, PDX3-1 (547 bp), shared no homologous regions in the genomes of PD-Temecula and CVC-9a5c, but was homologous to two regions in ALSD-Dixon and one in OLSD-Ann-1. PDX3-1 contained the C-terminal portion of an integrase gene found in Enterobacteriaceae phages. A 43-bp sequence at the 3' end of PDX3-1 was almost identical to part of the tRNA-lys gene, which could be the att site for DNA integration.