Submitted to: International Conference on Mycorrhiza
Publication Type: Abstract Only
Publication Acceptance Date: 4/21/2003
Publication Date: 8/10/2003
Citation: ALLEN, J.W., PFEFFER, P.E., JIN, H., SHACHAR-HILL, Y. LOCATING THE SITE OF DNA SYNTHESIS AND MEASURING FLUX THROUGH THE PENTOSE PHOSPHATE PATHWAY IN AN ARBUSCULAR MYCORRHIZAL FUNGUS. INTERNATIONAL CONFERENCE ON MYCORRHIZA. 2003. ABSTRACT #249. p. 288.
Technical Abstract: The pentose phosphate pathway (PPP) is both an important source of reducing equivalents for lipid synthesis and the major pathway by which pentose is synthesized de novo for the production of nucleotides. This has allowed us to develop a novel means to simultaneously locate the synthesis of DNA and determine the flux through the PPP. The method is based on analysis by NMR spectroscopy, GC-MS, and flux modeling of 13C labeling patterns in the deoxyribosyl moieties of extracted DNA after exposure to labeled precursors. This method was demonstrated using E.coli, then applied to the mycorrhizal fungus Glomus intraradices in an in vitro split-plate culturing system. The potential usefulness of this method as well as the information on the site of DNA synthesis and PPP fluxes is discussed in the context of the metabolism and life cycle of arbuscular mycorrhizal fungi.