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Title: DEVELOPMENT OF A NECLEOCAPSID BASED PEPTIDE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF AVIAN METAPNEUMOVIRUS ANTIBODIES

Author
item Alvarez, Rene
item NJENGA, M KARIUKI - UNIV OF MN-ST PAUL, MN
item Seal, Bruce

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 7/19/2003
Publication Date: 7/19/2003
Citation: Alvarez, R., Njenga, M., Seal, B.S. 2003. Development of a necleocapsid based peptide enzyme-linked immunosorbent assay for detection of avian metapneumovirus antibodies. American Association of Avian Pathologists. 2003 CDROM.

Interpretive Summary:

Technical Abstract: The avian metapneumovirus (aMPV) nucleocapsid (N) protein sequences of serotype A, B, and C were aligned for comparative sequence analysis. Five aMPV-specific peptides were synthesized for the development of a peptide-antigen, enzyme-linked immunosorbent assay (aMPV/N-peptide ELISA) to detect aMPV-specific antibodies among turkeys. Serum from naturally- and experimentally-infected turkeys were utilized to demonstrate the presence of antibodies reactive to the chemically synthesized aMPV N-peptides. Data obtained with the peptide-based ELISA correlated positively with total aMPV viral antigen based ELISAs. The aMPV N-peptide 1 (DLSYKHAILKESQYTIKRDV) with variations at only three amino acids among aMPV serotypes was evalutated as an universal aMPV ELISA antigen. Sera from turkeys infected with aMPV serotypes A, B, and C reacted positive with this antigen. These results indicated that aMPV N-peptide 1 can be utilized as an universal ELISA antigen for all aMPV sertotypes. Also, this peptide may potentially be utilized for serological detection of antibodies to the recently identified human metapneumovirus that possesses a 100% sequence identity within this portion of the N protein of aMPV/C.