Submitted to: International Meeting on Plant and Microbe Adaptations to Cold
Publication Type: Abstract Only
Publication Acceptance Date: 4/16/2003
Publication Date: 6/1/2003
Citation: WISNIEWSKI, M.E., BASSETT, C.L., FARRELL, R., ARTLIP, T.S. TWO DEHYDRIN GENES IN PEACH BARK TISSUES:TRANSCRIPT ACCUMULATION IN RESPONSE TO ENVIRONMENTAL STRESS AND COMPARATIVE ANALYSIS OF THEIR PROMOTOERS. INTERNATIONAL MEETING ON PLANT AND MICROBE ADAPTATIONS TO COLD. JUNE 2003. Interpretive Summary:
Technical Abstract: We have previously reported on protein and transcript accumulation of a 60 kDa dehydrin (PCA60; PpDhn1) from peach and described its cryoprotective and antifreeze properties. Presently, we report on a second dehydrin gene (PpDhn2) from peach and present a comparison of the two dehydrin genes. Analysis of the genomic subclone on which the genes reside indicate a potential synteny with two dehydrin genes (Xero1 and Xero2) found on chromosome III in Arabidopsis. The predicted sequences of the two dehydrins contain some or all of the YSK signature motifs of dehydrins. In contrast to PpDhn1, PpDhn2 contains an intron, has a serine track, and a reduced number of K segments. The promoters of the two dehydrins both contain ABRE-like elements (ABA-inducible). PpDhn1 contains two C-repeats (CCGAC), which play a role in cold induction transcription via the CBF transcription factor, while a C-repeat was only noted in the complementary strand of PpDhn2. Analysis of transcript accumulation of the two genes indicates that they are both highly inducible by water deficit and low temperature but only slightly or not at all by short day photoperiod. Preliminary studies also indicate the potential of different transcript initiation sites for both dehydrin genes indicating another potential level of gene regulation.