Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 3/20/2003
Publication Date: 7/25/2003
Citation: Lin, H., Walker, A. 2003. Developing transcriptional profiles and microarray expression analysis of grape plant responses to Xylella fastidiosa [Abstract]. American Society of Plant Biology. p. 162.
Technical Abstract: Xylella fastidiosa (Xf) is a gram negative bacterium capable of causing serious disease in a number of plants including Pierce's Disease (PD) of grape (Vitis vinifera L.). The impact of PD on the California Viticulture industry has been exacerbated by the recent introduction and establishment of a more effective vector, Homalodisca coagulata, the glassy-winged sharpshooter. However, information regarding the molecular basis of the host plant response to Xf infection and resistance mechanisms involved is very limited. In order to characterize the molecular events in the grape/Xf interaction, we are developing a genomic approach to identify transcriptional pathways correlated with susceptibility and resistance. Highly resistant and susceptible genotypes were selected from a Vitis rupestris x Muscadinia rotundifolia population. Research is underway to construct reciprocal Suppression Subtractive Hybridization (SSH) cDNA libraries for both resistant and susceptible genotypes. These libraries will represent the complete spectrum of gene expression profiles in response to Xf infection. This strategy has proven to be an efficient methodology for cloning and identifying differentially expressed genes associated with signal recognition/transduction and complex interactions between pathogens and host plants. About 8,000 clones will be sequenced from SSH cDNA libraries. A subset of candidate genes based on BLAST search of the Arabidopsis database and publicly available grape ESTs, will be selected. Purified cDNAs from corresponding genes will be spotted on microarray slides for differential expression analysis. The information derived from this study will reveal the details of the transcriptional pathways involved in the grape responses to Xf infection.